› 5svd

X-ray diffraction
2.1Å resolution

Nop9, a new PUF-like protein, prevents premature pre-rRNA cleavage to correctly process mature 18S rRNA

Released:
DOI: 10.2210/pdb5svd/pdb
PDB entry 5svd coloured by chain, front view.
PDB entry 5svd coloured by chain, side view.
PDB entry 5svd coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA.
Zhang J, McCann KL, Qiu C, Gonzalez LE, Baserga SJ, Hall TM
Nat Commun 7 13085 (2016)
PMID: 27725644

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155594 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleolar protein 9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 600 amino acids
Theoretical weight: 69.95 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P47077 (Residues: 46-645; Coverage: 90%)
Gene names: J1357, NOP9, YJL010C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 67.501Å b: 110.466Å c: 114.846Å
α: 90° β: 92.22° γ: 90°
R-values:
R R work R free
0.186 0.185 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Post-transcriptional Regulatory Functions of Mammalian Pumilio Proteins.
Goldstrohm et al. (2018)
4 more

3 mentions without citation

Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA.
Zhang et al. (2016)
2 more