› 5p2p

X-ray diffraction
2.4Å resolution

X-RAY STRUCTURE OF PHOSPHOLIPASE A2 COMPLEXED WITH A SUBSTRATE-DERIVED INHIBITOR

Released:
DOI: 10.2210/pdb5p2p/pdb
PDB entry 5p2p coloured by chain, front view.
PDB entry 5p2p coloured by chain, side view.
PDB entry 5p2p coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
X-ray structure of phospholipase A2 complexed with a substrate-derived inhibitor.
Thunnissen MM, Ab E, Kalk KH, Drenth J, Dijkstra BW, Kuipers OP, Dijkman R, de Haas GH, Verheij HM
Nature 347 689-91 (1990)
PMID: 2215698

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2, major isoenzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 119 amino acids
Theoretical weight: 13.43 KDa
Source organism: Sus scrofa
Expression system: Not provided
UniProt:
  • Canonical: P00592 (Residues: 23-146; Coverage: 91%)
Gene name: PLA2G1B
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand DHG 2 x DHG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 52.57Å b: 62.44Å c: 85.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 not available not available
Expression system: Not provided

Quick links

Citations

13 review citations

The C2 domain calcium-binding motif: structural and functional diversity.
Nalefski et al. (1996)
12 more

11 mentions without citation

Flexibility and binding affinity in protein-ligand, protein-protein and multi-component protein interactions: limitations of current computational approaches.
Tuffery et al. (2012)
10 more