› 5oow

X-ray diffraction
2.9Å resolution

Crystal structure of lobe II from the nucleotide binding domain of DnaK in complex with AMPPCP

Released:
DOI: 10.2210/pdb5oow/pdb
PDB entry 5oow coloured by chain, front view.
PDB entry 5oow coloured by chain, side view.
PDB entry 5oow coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
A folding nucleus and minimal ATP binding domain of Hsp70 identified by single-molecule force spectroscopy.
Bauer D, Meinhold S, Jakob RP, Stigler J, Merkel U, Maier T, Rief M, Žoldák G
Proc Natl Acad Sci U S A 115 4666-4671 (2018)
PMID: 29669923

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperone protein DnaK Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 22.46 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6Y8 (Residues: 183-383; Coverage: 32%)
Gene names: JW0013, b0014, dnaK, groP, grpF, seg
Sequence domains: Hsp70 protein

Ligands and Environments

1 bound ligand:
Ligand ACP 2 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 59.857Å b: 64.203Å c: 74.639Å
α: 90° β: 113.31° γ: 90°
R-values:
R R work R free
0.239 0.239 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Single-Molecule Studies of Protein Folding with Optical Tweezers.
Bustamante et al. (2020)
2 more

2 mentions without citation

A folding nucleus and minimal ATP binding domain of Hsp70 identified by single-molecule force spectroscopy.
Bauer et al. (2018)
1 more