› 5oki

X-ray diffraction
4.5Å resolution

Crystal structure of the Ctf18-1-8 module from Ctf18-RFC in complex with a 63 kDa fragment of DNA Polymerase epsilon

Released:
DOI: 10.2210/pdb5oki/pdb
PDB entry 5oki coloured by chain, front view.
PDB entry 5oki coloured by chain, side view.
PDB entry 5oki coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural Basis for the Recruitment of Ctf18-RFC to the Replisome.
Grabarczyk DB, Silkenat S, Kisker C
Structure 26 137-144.e3 (2018)
PMID: 29225079

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-149481 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
DNA polymerase epsilon catalytic subunit A Chains: A, B
Molecule details ›
Chains: A, B
Length: 524 amino acids
Theoretical weight: 59.75 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21951 (Residues: 1-524; Coverage: 24%)
Gene names: DUN2, N0825, POL2, YNL262W
Sequence domains: DNA polymerase family B, exonuclease domain
Sister chromatid cohesion protein DCC1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 380 amino acids
Theoretical weight: 44.13 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25559 (Residues: 1-380; Coverage: 100%)
Gene names: DCC1, YCL016C, YCL16C
Sequence domains: Sister chromatid cohesion protein Dcc1
Chromosome transmission fidelity protein 8 Chains: D, H
Molecule details ›
Chains: D, H
Length: 133 amino acids
Theoretical weight: 15.19 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38877 (Residues: 1-133; Coverage: 100%)
Gene names: CTF8, YHR191C
Sequence domains: Ctf8
Chromosome transmission fidelity protein 18 Chains: E, I
Molecule details ›
Chains: E, I
Length: 26 amino acids
Theoretical weight: 3.17 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49956 (Residues: 715-740; Coverage: 4%)
Gene names: CHL12, CTF18, YM9582.03C, YMR078C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 121.678Å b: 145.826Å c: 378.752Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.294 0.293 0.312
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Eukaryotic clamp loaders and unloaders in the maintenance of genome stability.
Lee et al. (2020)
3 more

1 mention without citation

Ctf18-RFC and DNA Pol ϵ form a stable leading strand polymerase/clamp loader complex required for normal and perturbed DNA replication.
Stokes et al. (2020)