› 5ok0

X-ray diffraction
2.15Å resolution

Structure of the D10N mutant of beta-phosphoglucomutase from Lactococcus lactis trapped with native reaction intermediate beta-glucose 1,6-bisphosphate to 2.2A resolution.

Released:
DOI: 10.2210/pdb5ok0/pdb
PDB entry 5ok0 coloured by chain, front view.
PDB entry 5ok0 coloured by chain, side view.
PDB entry 5ok0 coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
van der Waals Contact between Nucleophile and Transferring Phosphorus Is Insufficient To Achieve Enzyme Transition-State Architecture
Johnson LA, Robertson AJ, Baxter NJ, Trevitt CR, Bisson C, Jin Y, Wood HP, Hounslow AM, Cliff MJ, Blackburn GM, Bowler MW, Waltho JP
ACS Catalysis (2018)

Function and Biology Details

Reaction catalysed: 
Beta-D-glucose 1-phosphate = beta-D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-phosphoglucomutase Chain: A
Molecule details ›
Chain: A
Length: 218 amino acids
Theoretical weight: 23.85 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P71447 (Residues: 1-218; Coverage: 99%)
Gene names: L0001, LL0429, pgmB
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand B16 1 x B16
Ligand MG 1 x MG
Ligand PDO 1 x PDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 36.81Å b: 54.94Å c: 103.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.22 0.292
Expression system: Escherichia coli BL21(DE3)

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