› 5of1

X-ray diffraction
1.56Å resolution

The structural versatility of TasA in B. subtilis biofilm formation

Released:
DOI: 10.2210/pdb5of1/pdb
PDB entry 5of1 coloured by chain, front view.
PDB entry 5of1 coloured by chain, side view.
PDB entry 5of1 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structural changes of TasA in biofilm formation of Bacillus subtilis.
Diehl A, Roske Y, Ball L, Chowdhury A, Hiller M, Molière N, Kramer R, Stöppler D, Worth CL, Schlegel B, Leidert M, Cremer N, Erdmann N, Lopez D, Stephanowitz H, Krause E, van Rossum BJ, Schmieder P, Heinemann U, Turgay K, Akbey Ü, Oschkinat H
Proc Natl Acad Sci U S A 115 3237-3242 (2018)
PMID: 29531041

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157033 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major biofilm matrix component Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 23.16 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P54507 (Residues: 30-239; Coverage: 90%)
Gene names: BSU24620, cotN, tasA, yqhF
Sequence domains: Camelysin metallo-endopeptidase

Ligands and Environments

2 bound ligands:
Ligand SAL 2 x SAL
Ligand EDO 3 x EDO
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P1
Unit cell:
a: 40.728Å b: 43.149Å c: 51.744Å
α: 89.92° β: 91.86° γ: 90.01°
R-values:
R R work R free
0.153 0.152 0.175
Expression system: Escherichia coli

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Citations

14 review citations

Bacillus subtilis biofilm formation and social interactions.
Arnaouteli et al. (2021)
13 more

8 mentions without citation

Structural changes of TasA in biofilm formation of Bacillus subtilis.
Diehl et al. (2018)
7 more