› 5obh

X-ray diffraction
2.4Å resolution

Crystal structure of glycine binding protein in complex with bicuculline

Released:
DOI: 10.2210/pdb5obh/pdb
PDB entry 5obh coloured by chain, front view.
PDB entry 5obh coloured by chain, side view.
PDB entry 5obh coloured by chain, top view.
Source organism: Aplysia californica
Primary publication:
A Structural Rationale for N-Methylbicuculline Acting as a Promiscuous Competitive Antagonist of Inhibitory Pentameric Ligand-Gated Ion Channels.
Jones MJ, Dawson A, Hales TG, Hunter WN
Chembiochem 21 1526-1533 (2020)
PMID: 31859406

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 249 amino acids
Theoretical weight: 28.31 KDa
Source organism: Aplysia californica
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8WSF8 (Residues: 1-236; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand J94 5 x J94
Ligand CL 5 x CL
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 71.243Å b: 132.236Å c: 132.631Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.23 0.249
Expression system: Spodoptera frugiperda

Quick links

Citations

1 citations in other articles

Delineating the activity of the potent nicotinic acetylcholine receptor agonists (+)-anatoxin-a and (-)-hosieine-A.
Parker et al. (2022)

1 mention without citation

A Structural Rationale for N-Methylbicuculline Acting as a Promiscuous Competitive Antagonist of Inhibitory Pentameric Ligand-Gated Ion Channels.
Jones et al. (2020)