› 5o4i

X-ray diffraction
1.8Å resolution

Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus dialyzed against solution containing 0.25 mM copper sulfate

Released:
DOI: 10.2210/pdb5o4i/pdb
PDB entry 5o4i coloured by chain, front view.
PDB entry 5o4i coloured by chain, side view.
PDB entry 5o4i coloured by chain, top view.
Source organism: Streptomyces griseoflavus
Primary publication:
[Incorporation of Copper Ions into T2/T3 Centers of Two-Domain Laccases].
Gabdulkhakov AG, Kostareva OS, Kolyadenko IA, Mikhaylina AO, Trubitsina LI, Tishchenko SV
Mol Biol (Mosk) 52 29-35
PMID: 29512633

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102518 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Two-domain laccase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 322 amino acids
Theoretical weight: 34.7 KDa
Source organism: Streptomyces griseoflavus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M4FJ81 (Residues: 1-322; Coverage: 100%)
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand CU 36 x CU
Ligand GOL 43 x GOL
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P1
Unit cell:
a: 76.933Å b: 94.931Å c: 116.587Å
α: 90.08° β: 90.2° γ: 91.65°
R-values:
R R work R free
0.18 0.177 0.222
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Engineering the Catalytic Properties of Two-Domain Laccase from Streptomyces griseoflavus Ac-993.
Kolyadenko et al. (2021)
3 more