› 5nxs

X-ray diffraction
4.19Å resolution

Crystal Structure of Human Pro-myostatin Precursor at 4.2 A Resolution with Experimental Phases from SeMet labelling

Released:
DOI: 10.2210/pdb5nxs/pdb
PDB entry 5nxs coloured by chain, front view.
PDB entry 5nxs coloured by chain, side view.
PDB entry 5nxs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human myostatin precursor and determinants of growth factor latency.
Cotton TR, Fischer G, Wang X, McCoy JC, Czepnik M, Thompson TB, Hyvönen M
EMBO J 37 367-383 (2018)
PMID: 29330193

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Growth/differentiation factor 8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 38.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14793 (Residues: 43-375; Coverage: 95%)
Gene names: GDF8, MSTN
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I23
Unit cell:
a: 196.83Å b: 196.83Å c: 196.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.276 0.274 0.301
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Structural biology of betaglycan and endoglin, membrane-bound co-receptors of the TGF-beta family.
Kim et al. (2019)
8 more

2 mentions without citation

Structure of the human myostatin precursor and determinants of growth factor latency.
Cotton et al. (2018)
1 more