› 5njp

X-ray diffraction
1.7Å resolution

Mix-and-diffuse serial synchrotron crystallography: structure of N,N',N''-Triacetylchitotriose bound to Lysozyme with 1s time-delay, phased with 1HEW

Released:
DOI: 10.2210/pdb5njp/pdb
PDB entry 5njp coloured by chain, front view.
PDB entry 5njp coloured by chain, side view.
PDB entry 5njp coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Mix-and-diffuse serial synchrotron crystallography.
Beyerlein KR, Dierksmeyer D, Mariani V, Kuhn M, Sarrou I, Ottaviano A, Awel S, Knoska J, Fuglerud S, Jönsson O, Stern S, Wiedorn MO, Yefanov O, Adriano L, Bean R, Burkhardt A, Fischer P, Heymann M, Horke DA, Jungnickel KEJ, Kovaleva E, Lorbeer O, Metz M, Meyer J, Morgan A, Pande K, Panneerselvam S, Seuring C, Tolstikova A, Lieske J, Aplin S, Roessle M, White TA, Chapman HN, Meents A, Oberthuer D
IUCrJ 4 769-777 (2017)
PMID: 29123679

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand CL 2 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P43212
Unit cell:
a: 79.61Å b: 79.61Å c: 38.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.201

Quick links

Citations

7 review citations

X-Ray Free-Electron Lasers for the Structure and Dynamics of Macromolecules.
Chapman HN. (2019)
6 more

1 mention without citation

Mix-and-diffuse serial synchrotron crystallography.
Beyerlein et al. (2017)