5n8o

Electron Microscopy
3.9Å resolution

Cryo EM structure of the conjugative relaxase TraI of the F/R1 plasmid system

Released:

Function and Biology Details

Reactions catalysed:
ATP + H(2)O = ADP + phosphate
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-119046 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Multifunctional conjugation protein TraI Chain: A
Molecule details ›
Chain: A
Length: 1756 amino acids
Theoretical weight: 192 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P14565 (Residues: 1-1756; Coverage: 100%)
Gene names: ECOK12F104, traI
Sequence domains:
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 22 nucleotides
Theoretical weight: 6.65 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-3601
Expression systems:
  • Escherichia coli
  • Not provided