› 5n6a

X-ray diffraction
3.1Å resolution

Cardiac muscle myosin motor domain in the pre-powerstroke state

Released:
DOI: 10.2210/pdb5n6a/pdb
PDB entry 5n6a coloured by chain, front view.
PDB entry 5n6a coloured by chain, side view.
PDB entry 5n6a coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Mechanistic and structural basis for activation of cardiac myosin force production by omecamtiv mecarbil.
Planelles-Herrero VJ, Hartman JJ, Robert-Paganin J, Malik FI, Houdusse A
Nat Commun 8 190 (2017)
PMID: 28775348

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin-7 Chain: A
Molecule details ›
Chain: A
Length: 828 amino acids
Theoretical weight: 94.79 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q9BE39 (Residues: 1-828; Coverage: 43%)
Gene name: MYH7
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ADP 1 x ADP
Ligand PO4 1 x PO4
Ligand MG 1 x MG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: C2221
Unit cell:
a: 87.66Å b: 149.83Å c: 154.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.247 0.32

Quick links

Citations

27 review citations

Three perspectives on the molecular basis of hypercontractility caused by hypertrophic cardiomyopathy mutations.
Spudich JA. (2019)
26 more

10 mentions without citation

The Central Role of the F-Actin Surface in Myosin Force Generation.
Doran et al. (2021)
9 more