› 5mvv

X-ray diffraction
1.4Å resolution

Crystal structure of Plasmodium falciparum actin I- gelsolin segment 1 -CdATP complex

Released:
DOI: 10.2210/pdb5mvv/pdb
PDB entry 5mvv coloured by chain, front view.
PDB entry 5mvv coloured by chain, side view.
PDB entry 5mvv coloured by chain, top view.
Source organisms:
Primary publication:
Rapid cadmium SAD phasing at the standard wavelength (1 Å).
Panneerselvam S, Kumpula EP, Kursula I, Burkhardt A, Meents A
Acta Crystallogr D Struct Biol 73 581-590 (2017)
PMID: 28695858

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146438 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin-1 Chain: A
Molecule details ›
Chain: A
Length: 378 amino acids
Theoretical weight: 42.05 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8I4X0 (Residues: 1-376; Coverage: 100%)
Gene names: ACT1, ACTI, PF3D7_1246200, PFL2215w
Sequence domains: Actin
Structure domains:
Gelsolin Chain: G
Molecule details ›
Chain: G
Length: 127 amino acids
Theoretical weight: 14.21 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13020 (Residues: 50-174; Coverage: 17%)
Gene names: Gsb, Gsn
Sequence domains: Gelsolin repeat
Structure domains: Severin

Ligands and Environments

4 bound ligands:
Ligand ATP 1 x ATP
Ligand SCN 1 x SCN
Ligand CD 3 x CD
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P21212
Unit cell:
a: 110.42Å b: 68.92Å c: 71.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.129 0.159
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Atomic view into Plasmodium actin polymerization, ATP hydrolysis, and fragmentation.
Kumpula et al. (2019)
1 more

1 mention without citation

Rapid cadmium SAD phasing at the standard wavelength (1 Å).
Panneerselvam et al. (2017)