› 5msr

X-ray diffraction
2.37Å resolution

Structure of the unmodified PCP-R domain of carboxylic acid reductase (CAR) from Segniliparus rugosus in complex with NADPH, P43 form

Released:
DOI: 10.2210/pdb5msr/pdb
PDB entry 5msr coloured by chain, front view.
PDB entry 5msr coloured by chain, side view.
PDB entry 5msr coloured by chain, top view.
Source organism: Segniliparus rugosus
Primary publication:
Structures of carboxylic acid reductase reveal domain dynamics underlying catalysis.
Gahloth D, Dunstan MS, Quaglia D, Klumbys E, Lockhart-Cairns MP, Hill AM, Derrington SR, Scrutton NS, Turner NJ, Leys D
Nat Chem Biol 13 975-981 (2017)
PMID: 28719588

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxylic acid reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1188 amino acids
Theoretical weight: 128.37 KDa
Source organism: Segniliparus rugosus
Expression system: Escherichia coli
UniProt:
  • Canonical: E5XP76 (Residues: 1-1188; Coverage: 100%)
Gene names: HMPREF9336_01297, car
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NAP 4 x NAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43
Unit cell:
a: 94.943Å b: 94.943Å c: 335.506Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.241
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Biocatalysis: Enzymatic Synthesis for Industrial Applications.
Wu et al. (2021)
10 more

3 mentions without citation

Natural products from thioester reductase containing biosynthetic pathways.
Mullowney et al. (2018)
2 more