5moc

X-ray diffraction
1.8Å resolution

Crystal structure of 14-3-3sigma and a p53 C-terminal 12-mer synthetic phosphopeptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-138132 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Cellular tumor antigen p53 Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P04637 (Residues: 382-393; Coverage: 3%)
Gene names: P53, TP53

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: C2221
Unit cell:
a: 81.91Å b: 112.27Å c: 62.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.208
Expression systems:
  • Escherichia coli
  • Not provided