› 5mmy

X-ray diffraction
1.88Å resolution

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase OXA-10 Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 27.64 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14489 (Residues: 20-264; Coverage: 99%)
Gene names: bla, oxa10, pse2
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

6 bound ligands:
Ligand EPE 1 x EPE
Ligand DMS 1 x DMS
Ligand GOL 4 x GOL
Ligand EDO 1 x EDO
Ligand CL 1 x CL
Ligand CO3 1 x CO3
1 modified residue:
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 48.772Å b: 102.189Å c: 127.409Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

β-Lactamases and β-Lactamase Inhibitors in the 21st Century.
Tooke et al. (2019)
2 more