› 5miu

X-ray diffraction
3.5Å resolution

G307E variant of Murine Apoptosis Inducing Factor (oxidized state)

Released:
DOI: 10.2210/pdb5miu/pdb
PDB entry 5miu coloured by chain, front view.
PDB entry 5miu coloured by chain, side view.
PDB entry 5miu coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural bases of the altered catalytic properties of a pathogenic variant of apoptosis inducing factor.
Sorrentino L, Cossu F, Milani M, Aliverti A, Mastrangelo E
Biochem Biophys Res Commun 490 1011-1017 (2017)
PMID: 28666871

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195532 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apoptosis-inducing factor 1, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 535 amino acids
Theoretical weight: 58.4 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Z0X1 (Residues: 78-612; Coverage: 87%)
Gene names: Aif, Aifm1, Pdcd8
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FAD 2 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 96.04Å b: 110.06Å c: 119.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.279
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Development of Novel Experimental Models to Study Flavoproteome Alterations in Human Neuromuscular Diseases: The Effect of Rf Therapy.
Tolomeo et al. (2020)
2 more

2 mentions without citation

Middle East respiratory syndrome coronavirus: another zoonotic betacoronavirus causing SARS-like disease.
Chan et al. (2015)
1 more