› 5mcl

X-ray diffraction
2Å resolution

Radiation damage to GH7 Family Cellobiohydrolase from Daphnia pulex: Dose (DWD) 18.4 MGy

Released:
DOI: 10.2210/pdb5mcl/pdb
PDB entry 5mcl coloured by chain, front view.
PDB entry 5mcl coloured by chain, side view.
PDB entry 5mcl coloured by chain, top view.
Source organism: Daphnia pulex
Primary publication:
OH cleavage from tyrosine: debunking a myth.
Bury CS, Carmichael I, Garman EF
J Synchrotron Radiat 24 7-18 (2017)
PMID: 28009542

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cellulose 1,4-beta-cellobiosidase (non-reducing end) Chains: A, B
Molecule details ›
Chains: A, B
Length: 445 amino acids
Theoretical weight: 48.23 KDa
Source organism: Daphnia pulex
Expression system: Trichoderma reesei QM6a
UniProt:
  • Canonical: E9G5J5 (Residues: 20-464; Coverage: 100%)
Gene names: CEL7A, DAPPUDRAFT_347598
Sequence domains: Glycosyl hydrolase family 7
Structure domains: Glycoside hydrolase, family 7, domain

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand SO4 1 x SO4
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 127.635Å b: 46.785Å c: 174.713Å
α: 90° β: 108.22° γ: 90°
R-values:
R R work R free
0.245 0.244 0.265
Expression system: Trichoderma reesei QM6a

Quick links

Citations

1 review citation

Atomic displacement parameters in structural biology.
Carugo O. (2018)
4 other articles

1 mention without citation

OH cleavage from tyrosine: debunking a myth.
Bury et al. (2017)