› 5mca

X-ray diffraction
1.6Å resolution

Crystal structure of FimH-LD R60P variant in the apo state

Released:
DOI: 10.2210/pdb5mca/pdb
PDB entry 5mca coloured by chain, front view.
PDB entry 5mca coloured by chain, side view.
PDB entry 5mca coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Conformational switch of the bacterial adhesin FimH in the absence of the regulatory domain: Engineering a minimalistic allosteric system.
Rabbani S, Fiege B, Eris D, Silbermann M, Jakob RP, Navarra G, Maier T, Ernst B
J Biol Chem 293 1835-1849 (2018)
PMID: 29180452

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140004 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type 1 fimbrin D-mannose specific adhesin Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 16.91 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08191 (Residues: 22-180; Coverage: 57%)
Gene names: JW4283, b4320, fimH
Sequence domains: FimH, mannose binding
Structure domains: Fimbrial-type adhesion domain

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 49.64Å b: 57.193Å c: 64.566Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.147 0.175
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Glycomimetics for the inhibition and modulation of lectins.
Leusmann et al. (2023)
1 more

6 mentions without citation

5-hydroxymethylcytosine accumulation in postmitotic neurons results in functional demethylation of expressed genes.
Mellén et al. (2017)
5 more