› 5m05

X-ray diffraction
2.68Å resolution

Chicken smooth muscle myosin motor domain co-crystallized with the specific CK-571 inhibitor, MgADP form

Released:
DOI: 10.2210/pdb5m05/pdb
PDB entry 5m05 coloured by chain, front view.
PDB entry 5m05 coloured by chain, side view.
PDB entry 5m05 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Highly selective inhibition of myosin motors provides the basis of potential therapeutic application.
Sirigu S, Hartman JJ, Planelles-Herrero VJ, Ropars V, Clancy S, Wang X, Chuang G, Qian X, Lu PP, Barrett E, Rudolph K, Royer C, Morgan BP, Stura EA, Malik FI, Houdusse AM
Proc Natl Acad Sci U S A 113 E7448-E7455 (2016)
PMID: 27815532

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin-11 Chain: A
Molecule details ›
Chain: A
Length: 800 amino acids
Theoretical weight: 91.44 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10587 (Residues: 1-790; Coverage: 40%)
Gene name: MYH11
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand 52E 1 x 52E
Ligand MG 1 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 73.19Å b: 202.97Å c: 67.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.244
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Three perspectives on the molecular basis of hypercontractility caused by hypertrophic cardiomyopathy mutations.
Spudich JA. (2019)
1 more

3 mentions without citation

Highly selective inhibition of myosin motors provides the basis of potential therapeutic application.
Sirigu et al. (2016)
2 more