› 5m04

X-ray diffraction
1.85Å resolution

Structure of ObgE from Escherichia coli

Released:
DOI: 10.2210/pdb5m04/pdb
PDB entry 5m04 coloured by chain, front view.
PDB entry 5m04 coloured by chain, side view.
PDB entry 5m04 coloured by chain, top view.
Source organism: Escherichia coli DH5[alpha]
Primary publication:
Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence.
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
J Biol Chem 292 5871-5883 (2017)
PMID: 28223358

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154837 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase ObgE/CgtA Chain: A
Molecule details ›
Chain: A
Length: 360 amino acids
Theoretical weight: 39.2 KDa
Source organism: Escherichia coli DH5[alpha]
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42641 (Residues: 1-340; Coverage: 87%)
Gene names: JW3150, b3183, cgtA, obg, obgE, yhbZ
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: C2221
Unit cell:
a: 64.59Å b: 83Å c: 177.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Regulatory Hierarchies Controlling Virulence Gene Expression in Shigella flexneri and Vibrio cholerae.
Dorman et al. (2018)
2 more

4 mentions without citation

The Impact of the Stringent Response on TRAFAC GTPases and Prokaryotic Ribosome Assembly.
Bennison et al. (2019)
3 more