› 5lv2

X-ray diffraction
2.29Å resolution

Crystal structure of mouse CARM1 in complex with inhibitor LH1246

Released:
DOI: 10.2210/pdb5lv2/pdb
PDB entry 5lv2 coloured by chain, front view.
PDB entry 5lv2 coloured by chain, side view.
PDB entry 5lv2 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Hijacking DNA methyltransferase transition state analogues to produce chemical scaffolds for PRMT inhibitors.
Halby L, Marechal N, Pechalrieu D, Cura V, Franchini DM, Faux C, Alby F, Troffer-Charlier N, Kudithipudi S, Jeltsch A, Aouadi W, Decroly E, Guillemot JC, Page P, Ferroud C, Bonnefond L, Guianvarc'h D, Cavarelli J, Arimondo PB
Philos Trans R Soc Lond B Biol Sci 373 (2018)
PMID: 29685976

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194407 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-arginine methyltransferase CARM1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 361 amino acids
Theoretical weight: 40.85 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9WVG6 (Residues: 130-487; Coverage: 59%)
Gene names: Carm1, Prmt4
Sequence domains: Ribosomal protein L11 methyltransferase (PrmA)
Structure domains:

Ligands and Environments

8 bound ligands:
Ligand SAO 4 x SAO
Ligand EDO 13 x EDO
Ligand LHB 4 x LHB
Ligand PG4 4 x PG4
Ligand M2M 2 x M2M
Ligand DXE 1 x DXE
Ligand PEG 1 x PEG
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 98.684Å b: 74.832Å c: 206.639Å
α: 90° β: 90.53° γ: 90°
R-values:
R R work R free
0.207 0.205 0.238
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

The Roles of Host 5-Methylcytosine RNA Methyltransferases during Viral Infections.
Wnuk et al. (2020)
9 other articles

3 mentions without citation

Protein arginine methyltransferases: insights into the enzyme structure and mechanism at the atomic level.
Tewary et al. (2019)
2 more