› 5lst

X-ray diffraction
2.75Å resolution

Crystal structure of the human RecQL4 helicase.

Released:
DOI: 10.2210/pdb5lst/pdb
PDB entry 5lst coloured by chain, front view.
PDB entry 5lst coloured by chain, side view.
PDB entry 5lst coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structural and functional characterization of human RecQ4 reveals insights into its helicase mechanism.
Kaiser S, Sauer F, Kisker C
Nat Commun 8 15907 (2017)
PMID: 28653661

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131738 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase Q4 Chain: A
Molecule details ›
Chain: A
Length: 693 amino acids
Theoretical weight: 76.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O94761 (Residues: 427-1116; Coverage: 57%)
Gene names: RECQ4, RECQL4
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 131.001Å b: 131.001Å c: 96.344Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

New Insights Into DNA Helicases as Druggable Targets for Cancer Therapy.
Datta et al. (2018)
7 more

5 mentions without citation

TWINKLE and Other Human Mitochondrial DNA Helicases: Structure, Function and Disease.
Peter et al. (2020)
4 more