› 5lrs

X-ray diffraction
2.9Å resolution

The Transcriptional Regulator PrfA from Listeria Monocytogenes in complex with glutathione and a 30-bp operator PrfA-box motif

Released:
DOI: 10.2210/pdb5lrs/pdb
PDB entry 5lrs coloured by chain, front view.
PDB entry 5lrs coloured by chain, side view.
PDB entry 5lrs coloured by chain, top view.
Source organism: Listeria monocytogenes
Primary publication:
Structural basis for glutathione-mediated activation of the virulence regulatory protein PrfA in Listeria.
Hall M, Grundström C, Begum A, Lindberg MJ, Sauer UH, Almqvist F, Johansson J, Sauer-Eriksson AE
Proc Natl Acad Sci U S A 113 14733-14738 (2016)
PMID: 27930316

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Listeriolysin regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 27.33 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P22262 (Residues: 1-237; Coverage: 100%)
Gene names: lmo0200, prfA
Structure domains:
DNA (30-MER) Chain: C
Molecule details ›
Chain: C
Length: 30 nucleotides
Theoretical weight: 9.26 KDa
Source organism: Listeria monocytogenes
Expression system: Not provided
DNA (30-MER) Chain: D
Molecule details ›
Chain: D
Length: 30 nucleotides
Theoretical weight: 9.18 KDa
Source organism: Listeria monocytogenes
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GSH 2 x GSH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 78.961Å b: 78.961Å c: 265.224Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.248 0.279
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 review citations

Listeria monocytogenes: towards a complete picture of its physiology and pathogenesis.
Radoshevich et al. (2018)
13 more

2 mentions without citation

Structural basis for glutathione-mediated activation of the virulence regulatory protein PrfA in Listeria.
Hall et al. (2016)
1 more