› 5lpv

X-ray diffraction
2.7Å resolution

Crystal structure of the BRI1 kinase domain (865-1160) in complex with AMPPNP and Mn from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb5lpv/pdb
PDB entry 5lpv coloured by chain, front view.
PDB entry 5lpv coloured by chain, side view.
PDB entry 5lpv coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Bojar D, Martinez J, Hothorn M

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127636 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein BRASSINOSTEROID INSENSITIVE 1 Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 33.36 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O22476 (Residues: 865-1160; Coverage: 25%)
Gene names: At4g39400, BRI1, F23K16.30
Sequence domains: Protein kinase domain

Ligands and Environments

2 bound ligands:
Ligand ANP 1 x ANP
Ligand MN 2 x MN
2 modified residues:
Ligand TPO 1 x TPO
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P62
Unit cell:
a: 115.63Å b: 115.63Å c: 49.64Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Brassinosteroids: Multidimensional Regulators of Plant Growth, Development, and Stress Responses.
Nolan et al. (2020)
18 more

4 mentions without citation

Structural analysis of receptor-like kinase SOBIR1 reveals mechanisms that regulate its phosphorylation-dependent activation.
Wei et al. (2022)
3 more