› 5lpm

X-ray diffraction
1.5Å resolution

Crystal structure of the bromodomain of human Ep300 bound to the inhibitor XDM3d

Released:
DOI: 10.2210/pdb5lpm/pdb
PDB entry 5lpm coloured by chain, front view.
PDB entry 5lpm coloured by chain, side view.
PDB entry 5lpm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Beyond the BET Family: Targeting CBP/p300 with 4-Acyl Pyrroles.
Hügle M, Lucas X, Ostrovskyi D, Regenass P, Gerhardt S, Einsle O, Hau M, Jung M, Breit B, Günther S, Wohlwend D
Angew Chem Int Ed Engl 56 12476-12480 (2017)
PMID: 28766825

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone acetyltransferase p300 Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 13.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q09472 (Residues: 1048-1161; Coverage: 5%)
Gene names: EP300, P300
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

2 bound ligands:
Ligand 71Y 4 x 71Y
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 37.861Å b: 81.087Å c: 44.779Å
α: 90° β: 110.58° γ: 90°
R-values:
R R work R free
0.159 0.158 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Regulation of DNA replication-coupled histone gene expression.
Mei et al. (2017)
3 more

2 mentions without citation

Rab GTPases: Emerging Oncogenes and Tumor Suppressive Regulators for the Editing of Survival Pathways in Cancer.
Gopal Krishnan et al. (2020)
1 more