› 5ljp

X-ray diffraction
1.1Å resolution

E20K/I59A/E72K/I92A/D126K/A142V FLAVODOXIN FROM ANABAENA

Released:
DOI: 10.2210/pdb5ljp/pdb
PDB entry 5ljp coloured by chain, front view.
PDB entry 5ljp coloured by chain, side view.
PDB entry 5ljp coloured by chain, top view.
Source organism: Nostoc sp. PCC 7119
Primary publication:
Direct examination of the relevance for folding, binding and electron transfer of a conserved protein folding intermediate.
Lamazares E, Vega S, Ferreira P, Medina M, Galano-Frutos JJ, Martínez-Júlvez M, Velázquez-Campoy A, Sancho J
Phys Chem Chem Phys 19 19021-19031 (2017)
PMID: 28702545

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavodoxin Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 18.81 KDa
Source organism: Nostoc sp. PCC 7119
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A3E0 (Residues: 2-170; Coverage: 99%)
Gene name: isiB
Sequence domains: Flavodoxin
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand FMN 1 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 37.919Å b: 62.911Å c: 64.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.16 0.176
Expression system: Escherichia coli BL21

Quick links

Citations

3 citation in other articles

Long-chain flavodoxin FldX1 improves Paraburkholderia xenovorans LB400 tolerance to oxidative stress caused by paraquat and H2O2.
Rodríguez-Castro et al. (2019)
2 more