5lel

X-ray diffraction
3.1Å resolution

Crystal structure of DARPin-DARPin rigid fusion, variant DD_Off7_10_3G124 in complex with Maltose-binding Protein and Green Fluorescent Protein

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-199641 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DD_Off7_10_3G124 Chains: A, D, G
Molecule details ›
Chains: A, D, G
Length: 325 amino acids
Theoretical weight: 34.95 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12
Maltose/maltodextrin-binding periplasmic protein Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 395 amino acids
Theoretical weight: 43.17 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0AEX9 (Residues: 29-392; Coverage: 98%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Green fluorescent protein Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 247 amino acids
Theoretical weight: 28.22 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 235Å b: 103.14Å c: 156.84Å
α: 90° β: 131.35° γ: 90°
R-values:
R R work R free
0.261 0.258 0.306
Expression system: Escherichia coli K-12