› 5l2r

X-ray diffraction
2.05Å resolution

Crystal structure of fumarate hydratase from Leishmania major

Released:
DOI: 10.2210/pdb5l2r/pdb
PDB entry 5l2r coloured by chain, front view.
PDB entry 5l2r coloured by chain, side view.
PDB entry 5l2r coloured by chain, top view.
Source organism: Leishmania major
Primary publication:
Crystal structure of an Fe-S cluster-containing fumarate hydratase enzyme from Leishmania major reveals a unique protein fold.
Feliciano PR, Drennan CL, Nonato MC
Proc Natl Acad Sci U S A 113 9804-9 (2016)
PMID: 27528683

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123143 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 604 amino acids
Theoretical weight: 66.51 KDa
Source organism: Leishmania major
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E9AE57 (Residues: 1-568; Coverage: 100%)
Gene names: FH2, LMJF_29_1960
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand LMR 4 x LMR
Ligand MLA 4 x MLA
Ligand GOL 8 x GOL
Ligand SF4 2 x SF4
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 65.675Å b: 85.128Å c: 241.476Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.177
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Aurothiomalate-Based Drugs as Potentially Novel Agents Against Leishmania major: A Mini Review.
Davoodi et al. (2022)
7 other articles

4 mentions without citation

Crystal structure of an Fe-S cluster-containing fumarate hydratase enzyme from Leishmania major reveals a unique protein fold.
Feliciano et al. (2016)
3 more