› 5ko6

X-ray diffraction
1.42Å resolution

Crystal Structure of Isoform 2 of Purine Nucleoside Phosphorylase from Schistosoma mansoni in complex with cytosine and ribose-1-phosphate

Released:
DOI: 10.2210/pdb5ko6/pdb
PDB entry 5ko6 coloured by chain, front view.
PDB entry 5ko6 coloured by chain, side view.
PDB entry 5ko6 coloured by chain, top view.
Source organism: Schistosoma mansoni
Primary publication:
The molecular structure of Schistosoma mansoni PNP isoform 2 provides insights into the nucleoside selectivity of PNPs.
Torini JR, Romanello L, Batista FAH, Serrão VHB, Faheem M, Zeraik AE, Bird L, Nettleship J, Reddivari Y, Owens R, DeMarco R, Borges JC, Brandão-Neto J, Pereira HD
PLoS One 13 e0203532 (2018)
PMID: 30192840

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 31.36 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0U3AGT1 (Residues: 1-287; Coverage: 100%)
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand CYT 1 x CYT
Ligand R1P 1 x R1P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P213
Unit cell:
a: 98.64Å b: 98.64Å c: 98.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.178
Expression system: Escherichia coli

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Citations

2 citation in other articles

In Silico Study of Cucurbita maxima Compounds as Potential Therapeutics Against Schistosomiasis.
Mtemeli et al. (2022)
1 more

1 mention without citation

The molecular structure of Schistosoma mansoni PNP isoform 2 provides insights into the nucleoside selectivity of PNPs.
Torini et al. (2018)