› 5kdw

X-ray diffraction
1.85Å resolution

IMPa metallopeptidase from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5kdw/pdb
PDB entry 5kdw coloured by chain, front view.
PDB entry 5kdw coloured by chain, side view.
PDB entry 5kdw coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Recognition of protein-linked glycans as a determinant of peptidase activity.
Noach I, Ficko-Blean E, Pluvinage B, Stuart C, Jenkins ML, Brochu D, Buenbrazo N, Wakarchuk W, Burke JE, Gilbert M, Boraston AB
Proc Natl Acad Sci U S A 114 E679-E688 (2017)
PMID: 28096352

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunomodulating metalloprotease Chains: A, B
Molecule details ›
Chains: A, B
Length: 885 amino acids
Theoretical weight: 96.38 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q9I5W4 (Residues: 42-923; Coverage: 100%)
Gene names: PA0572, impA
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand EDO 42 x EDO
Ligand ZN 2 x ZN
Ligand PO4 7 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 98.92Å b: 132.73Å c: 103.07Å
α: 90° β: 103.81° γ: 90°
R-values:
R R work R free
0.155 0.153 0.186
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

10 review citations

Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier.
Martens et al. (2018)
9 more

5 mentions without citation

O-Mucin-degrading carbohydrate-active enzymes and their possible implication in inflammatory bowel diseases.
Labourel et al. (2023)
4 more