5k5q

X-ray diffraction
2.65Å resolution

Structure of AspA-DNA complex: novel centromere bindng protein-centromere complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-118896 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH arsR-type domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 92 amino acids
Theoretical weight: 10.6 KDa
Source organism: Sulfolobus sp. NOB8H2
Expression system: Escherichia coli
UniProt:
  • Canonical: O93706 (Residues: 2-93; Coverage: 99%)
Sequence domains: Sugar-specific transcriptional regulator TrmB
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (32-MER) Chain: P
Molecule details ›
Chain: P
Length: 32 nucleotides
Theoretical weight: 9.83 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (32-MER) Chain: N
Molecule details ›
Chain: N
Length: 32 nucleotides
Theoretical weight: 9.85 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 154.65Å b: 56.92Å c: 103.8Å
α: 90° β: 112.3° γ: 90°
R-values:
R R work R free
0.262 0.26 0.28
Expression systems:
  • Escherichia coli
  • Not provided