› 5jzl

X-ray diffraction
1.8Å resolution

The Structure of Monomeric Ultra Stable Green Fluorescent Protein

Released:
DOI: 10.2210/pdb5jzl/pdb
PDB entry 5jzl coloured by chain, front view.
PDB entry 5jzl coloured by chain, side view.
PDB entry 5jzl coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
A Novel Ultra-Stable, Monomeric Green Fluorescent Protein For Direct Volumetric Imaging of Whole Organs Using CLARITY.
Scott DJ, Gunn NJ, Yong KJ, Wimmer VC, Veldhuis NA, Challis LM, Haidar M, Petrou S, Bathgate RAD, Griffin MDW
Sci Rep 8 667 (2018)
PMID: 29330459

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 28.08 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 3-238; Coverage: 99%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand NA 2 x NA
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 47.352Å b: 95.706Å c: 59.649Å
α: 90° β: 104.21° γ: 90°
R-values:
R R work R free
0.177 0.175 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Principles and characteristics of biological assemblies in experimentally determined protein structures.
Xu et al. (2019)
3 more

2 mentions without citation

A Novel Ultra-Stable, Monomeric Green Fluorescent Protein For Direct Volumetric Imaging of Whole Organs Using CLARITY.
Scott et al. (2018)
1 more