› 5jyf

X-ray diffraction
2.62Å resolution

Structures of Streptococcus agalactiae GBS GAPDH in different enzymatic states

Released:
DOI: 10.2210/pdb5jyf/pdb
PDB entry 5jyf coloured by chain, front view.
PDB entry 5jyf coloured by chain, side view.
PDB entry 5jyf coloured by chain, top view.
Source organism: Streptococcus agalactiae
Primary publication:
Crystal Structures of Group B Streptococcus Glyceraldehyde-3-Phosphate Dehydrogenase: Apo-Form, Binary and Ternary Complexes.
Schormann N, Ayres CA, Fry A, Green TJ, Banerjee S, Ulett GC, Chattopadhyay D
PLoS One 11 e0165917 (2016)
PMID: 27875551

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189588 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 356 amino acids
Theoretical weight: 38.21 KDa
Source organism: Streptococcus agalactiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ALW2 (Residues: 1-336; Coverage: 100%)
Gene names: AX245_09885, C4618_13560, C6N07_02390, NCTC8184_00328, gap, gapA
Sequence domains:
Structure domains: Dihydrodipicolinate Reductase; domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 79.215Å b: 112.634Å c: 147.633Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.278
Expression system: Escherichia coli

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Citations

6 citation in other articles

CHARMM-GUI ligand reader and modeler for CHARMM force field generation of small molecules.
Kim et al. (2017)
5 more

2 mentions without citation

Epitopes identified in GAPDH from Clostridium difficile recognized as common antigens with potential autoimmunizing properties.
Razim et al. (2018)
1 more