› 5jli

X-ray diffraction
1.55Å resolution

Nitric oxide complex of the L16A mutant of cytochrome c prime from Alcaligenes xylosoxidans

Released:
DOI: 10.2210/pdb5jli/pdb
PDB entry 5jli coloured by chain, front view.
PDB entry 5jli coloured by chain, side view.
PDB entry 5jli coloured by chain, top view.
Source organism: Achromobacter xylosoxidans
Primary publication:
Engineering proximal vs. distal heme-NO coordination via dinitrosyl dynamics: implications for NO sensor design.
Kekilli D, Petersen CA, Pixton DA, Ghafoor DD, Abdullah GH, Dworkowski FSN, Wilson MT, Heyes DJ, Hardman SJO, Murphy LM, Strange RW, Scrutton NS, Andrew CR, Hough MA
Chem Sci 8 1986-1994 (2017)
PMID: 28451315

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c' Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 13.59 KDa
Source organism: Achromobacter xylosoxidans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00138 (Residues: 1-127; Coverage: 100%)
Sequence domains: Cytochrome C'
Structure domains: Cytochrome c/b562

Ligands and Environments

4 bound ligands:
Ligand HEC 1 x HEC
Ligand ASC 1 x ASC
Ligand SO4 2 x SO4
Ligand NO 1 x NO
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6522
Unit cell:
a: 53.629Å b: 53.629Å c: 180.523Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.17 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

One fold, two functions: cytochrome P460 and cytochrome c'-β from the methanotroph Methylococcus capsulatus (Bath).
Adams et al. (2019)
4 more