› 5jeq

X-ray diffraction
1.9Å resolution

Fragment of nitrate/nitrite sensor histidine kinase NarQ (R50K) in symmetric apo state

Released:
DOI: 10.2210/pdb5jeq/pdb
PDB entry 5jeq coloured by chain, front view.
PDB entry 5jeq coloured by chain, side view.
PDB entry 5jeq coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mechanism of transmembrane signaling by sensor histidine kinases.
Gushchin I, Melnikov I, Polovinkin V, Ishchenko A, Yuzhakova A, Buslaev P, Bourenkov G, Grudinin S, Round E, Balandin T, Borshchevskiy V, Willbold D, Leonard G, Büldt G, Popov A, Gordeliy V
Science 356 (2017)
PMID: 28522691

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrate/nitrite sensor protein NarQ Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P27896 (Residues: 1-230; Coverage: 41%)
Gene names: JW2453, b2469, narQ
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I212121
Unit cell:
a: 39.593Å b: 59.366Å c: 239.583Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.285
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Multidrug efflux pumps: structure, function and regulation.
Du et al. (2018)
12 more

8 mentions without citation

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
7 more