› 5ja2

X-ray diffraction
3Å resolution

EntF, a Terminal Nonribosomal Peptide Synthetase Module Bound to the non-Native MbtH-Like Protein PA2412

Released:
DOI: 10.2210/pdb5ja2/pdb
PDB entry 5ja2 coloured by chain, front view.
PDB entry 5ja2 coloured by chain, side view.
PDB entry 5ja2 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of a Nonribosomal Peptide Synthetase Module Bound to MbtH-like Proteins Support a Highly Dynamic Domain Architecture.
Miller BR, Drake EJ, Shi C, Aldrich CC, Gulick AM
J Biol Chem 291 22559-22571 (2016)
PMID: 27597544

Function and Biology Details

Reactions catalysed: 
(1a) ATP + L-serine = diphosphate + (L-seryl)adenylate
6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine = enterobactin + 6 AMP + 6 diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145845 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Enterobactin synthase component F Chain: A
Molecule details ›
Chain: A
Length: 1295 amino acids
Theoretical weight: 142.31 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11454 (Residues: 1-1293; Coverage: 100%)
Gene names: JW0578, b0586, entF
Sequence domains:
MbtH-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 8.65 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I169 (Residues: 1-72; Coverage: 100%)
Gene name: PA2412
Sequence domains: MbtH-like protein

Ligands and Environments

2 bound ligands:
Ligand 75C 1 x 75C
Ligand BU9 2 x BU9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Unit cell:
a: 159.27Å b: 57.69Å c: 182.424Å
α: 90° β: 97.62° γ: 90°
R-values:
R R work R free
0.206 0.205 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Nonribosomal Peptide Synthesis-Principles and Prospects.
Süssmuth et al. (2017)
16 more

3 mentions without citation

Structural advances toward understanding the catalytic activity and conformational dynamics of modular nonribosomal peptide synthetases.
Patel et al. (2023)
2 more