› 5j8q

X-ray diffraction
1.7Å resolution

Crystal Structure of the Cysteine Desulfurase SufS of Bacillus subtilis

Released:
DOI: 10.2210/pdb5j8q/pdb
PDB entry 5j8q coloured by chain, front view.
PDB entry 5j8q coloured by chain, side view.
PDB entry 5j8q coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Crystal Structure of Bacillus subtilis Cysteine Desulfurase SufS and Its Dynamic Interaction with Frataxin and Scaffold Protein SufU.
Blauenburg B, Mielcarek A, Altegoer F, Fage CD, Linne U, Bange G, Marahiel MA
PLoS One 11 e0158749 (2016)
PMID: 27382962

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase SufS Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 45.98 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32164 (Residues: 1-406; Coverage: 100%)
Gene names: BSU32690, csd, sufS, yurW
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand ALA 1 x ALA
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 93.191Å b: 93.191Å c: 130.543Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.118 0.116 0.148
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Fe-S cluster biogenesis by the bacterial Suf pathway.
Blahut et al. (2020)
2 more

6 mentions without citation

Iron-sulfur clusters biogenesis by the SUF machinery: close to the molecular mechanism understanding.
Pérard et al. (2018)
5 more