5j84

X-ray diffraction
2.4Å resolution

Crystal structure of L-arabinonate dehydratase in holo-form

Released:

Function and Biology Details

Reactions catalysed:
L-arabinonate = 2-dehydro-3-deoxy-L-arabinonate + H(2)O
D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H(2)O
D-fuconate = 2-dehydro-3-deoxy-D-fuconate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-110304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-arabinonate dehydratase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 588 amino acids
Theoretical weight: 63.86 KDa
Source organism: Rhizobium leguminosarum bv. trifolii WSM2304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B5ZZ34 (Residues: 2-579; Coverage: 100%)
Gene names: Rleg2_2909, araD
Sequence domains: Dehydratase family

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 106.07Å b: 208.61Å c: 147.09Å
α: 90° β: 90.43° γ: 90°
R-values:
R R work R free
0.155 0.156 0.185
Expression system: Escherichia coli BL21(DE3)