› 5j77

X-ray diffraction
2.1Å resolution

Mutant glyceraldehyde dehydrogenase (F34M+S405N) from Thermoplasma acidophilum

Released:
DOI: 10.2210/pdb5j77/pdb
PDB entry 5j77 coloured by chain, front view.
PDB entry 5j77 coloured by chain, side view.
PDB entry 5j77 coloured by chain, top view.
Source organism: Thermoplasma acidophilum DSM 1728
Entry authors: Iermak I, Mesters JR, Kuta Smatanova I

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde + NADP(+) + H(2)O = D-glycerate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190869 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-glyceraldehyde dehydrogenase (NADP(+)) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 508 amino acids
Theoretical weight: 56.53 KDa
Source organism: Thermoplasma acidophilum DSM 1728
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HK01 (Residues: 1-493; Coverage: 100%)
Gene name: Ta0809
Sequence domains: Aldehyde dehydrogenase family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2
Unit cell:
a: 108.071Å b: 158.423Å c: 130.05Å
α: 90° β: 91.57° γ: 90°
R-values:
R R work R free
0.178 0.176 0.226
Expression system: Escherichia coli BL21(DE3)

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