› 5j44

X-ray diffraction
2.91Å resolution

Crystal structure of the Secreted Extracellular protein A (SepA) from Shigella flexneri

Released:
DOI: 10.2210/pdb5j44/pdb
PDB entry 5j44 coloured by chain, front view.
PDB entry 5j44 coloured by chain, side view.
PDB entry 5j44 coloured by chain, top view.
Source organism: Shigella flexneri
Primary publication:
Shigella depends on SepA to destabilize the intestinal epithelial integrity via cofilin activation.
Maldonado-Contreras A, Birtley JR, Boll E, Zhao Y, Mumy KL, Toscano J, Ayehunie S, Reinecker HC, Stern LJ, McCormick BA
Gut Microbes 8 544-560 (2017)
PMID: 28598765

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease SepA Chains: A, B
Molecule details ›
Chains: A, B
Length: 1033 amino acids
Theoretical weight: 109.4 KDa
Source organism: Shigella flexneri
Expression system: Shigella flexneri
UniProt:
  • Canonical: Q8VSL2 (Residues: 57-1089; Coverage: 79%)
Gene names: CP0070, sepA
Sequence domains: Immunoglobulin A1 protease
Structure domains: Pectate Lyase C-like

Ligands and Environments

1 bound ligand:
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P3221
Unit cell:
a: 143.52Å b: 143.52Å c: 269.25Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.214 0.239
Expression system: Shigella flexneri

Quick links

Citations

10 review citations

Tight Junctions as a Key for Pathogens Invasion in Intestinal Epithelial Cells.
Paradis et al. (2021)
9 more

4 mentions without citation

Shigella depends on SepA to destabilize the intestinal epithelial integrity via cofilin activation.
Maldonado-Contreras et al. (2017)
3 more