› 5iyv

X-ray diffraction
2.56Å resolution

Crystal structure of the Arabidopsis receptor kinase HAESA LRR ectdomain in complex with the peptide hormone IDL1.

Released:
DOI: 10.2210/pdb5iyv/pdb
PDB entry 5iyv coloured by chain, front view.
PDB entry 5iyv coloured by chain, side view.
PDB entry 5iyv coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission.
Santiago J, Brandt B, Wildhagen M, Hohmann U, Hothorn LA, Butenko MA, Hothorn M
Elife 5 (2016)
PMID: 27058169

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155643 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Receptor-like protein kinase 5 Chain: A
Molecule details ›
Chain: A
Length: 616 amino acids
Theoretical weight: 67.22 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P47735 (Residues: 20-620; Coverage: 61%)
Gene names: At4g28490, F21O9.180, HAE, RLK5
Sequence domains:
Protein IDA-LIKE 1 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.31 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
UniProt:
  • Canonical: Q29PV4 (Residues: 67-78; Coverage: 20%)
Gene names: At3g25655, IDL1, T5M7

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 3 x NAG
Ligand EDO 1 x EDO
1 modified residue:
Ligand HYP 1 x HYP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 150.181Å b: 150.181Å c: 60.07Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.231
Expression systems:
  • Trichoplusia ni
  • Not provided

Quick links

Citations

27 review citations

Regulation of pattern recognition receptor signalling in plants.
Couto et al. (2016)
26 more