› 5ixr

X-ray diffraction
2.5Å resolution

Crystal Structure of C139A nostoc H-NOX domain with imidazole

Released:
DOI: 10.2210/pdb5ixr/pdb
PDB entry 5ixr coloured by chain, front view.
PDB entry 5ixr coloured by chain, side view.
PDB entry 5ixr coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Entry authors: Kumar V, van den Akker F

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme NO-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.36 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YUQ7 (Residues: 1-182; Coverage: 96%)
Gene name: alr2278
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

3 bound ligands:
Ligand HEM 2 x HEM
Ligand IMD 2 x IMD
Ligand MLI 2 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P213
Unit cell:
a: 123.051Å b: 123.051Å c: 123.051Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.131 0.174
Expression system: Escherichia coli

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