› 5ixj

X-ray diffraction
1.54Å resolution

Tryptophan Synthase beta-subunit from Pyrococcus furiosus with L-threonine non-covalently bound in the active site

Released:
DOI: 10.2210/pdb5ixj/pdb
PDB entry 5ixj coloured by chain, front view.
PDB entry 5ixj coloured by chain, side view.
PDB entry 5ixj coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Synthesis of β-Branched Tryptophan Analogues Using an Engineered Subunit of Tryptophan Synthase.
Herger M, van Roye P, Romney DK, Brinkmann-Chen S, Buller AR, Arnold FH
J Am Chem Soc 138 8388-91 (2016)
PMID: 27355405

Function and Biology Details

Reaction catalysed: 
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan synthase beta chain 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 396 amino acids
Theoretical weight: 43.89 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U093 (Residues: 1-388; Coverage: 100%)
Gene names: PF1706, trpB1
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand THR 4 x THR
Ligand NA 4 x NA
1 modified residue:
Ligand LLP 4 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 86.718Å b: 112.367Å c: 160.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.195
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Biocatalysis: Enzymatic Synthesis for Industrial Applications.
Wu et al. (2021)
5 more