› 5ius

X-ray diffraction
2.89Å resolution

Crystal structure of human PD-L1 in complex with high affinity PD-1 mutant

Released:
DOI: 10.2210/pdb5ius/pdb
PDB entry 5ius coloured by chain, front view.
PDB entry 5ius coloured by chain, side view.
PDB entry 5ius coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and Dynamics of PD-L1 and an Ultra-High-Affinity PD-1 Receptor Mutant.
Pascolutti R, Sun X, Kao J, Maute RL, Ring AM, Bowman GR, Kruse AC
Structure 24 1719-1728 (2016)
PMID: 27618663

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172199 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Programmed cell death protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15116 (Residues: 26-146; Coverage: 46%)
Gene names: PD1, PDCD1
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Programmed cell death 1 ligand 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 225 amino acids
Theoretical weight: 25.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZQ7 (Residues: 18-239; Coverage: 81%)
Gene names: B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P41
Unit cell:
a: 86.851Å b: 86.851Å c: 111.848Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.208 0.26
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The Diverse Function of PD-1/PD-L Pathway Beyond Cancer.
Qin et al. (2019)
9 more

14 mentions without citation

Immune Checkpoint PD-1/PD-L1: Is There Life Beyond Antibodies?
Konstantinidou et al. (2018)
13 more