› 5ikc

X-ray diffraction
2.06Å resolution

X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF HUMAN DOUBLECORTIN in complex with FAB

Released:
DOI: 10.2210/pdb5ikc/pdb
PDB entry 5ikc coloured by chain, front view.
PDB entry 5ikc coloured by chain, side view.
PDB entry 5ikc coloured by chain, top view.
Source organisms:
Primary publication:
Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies.
Burger D, Stihle M, Sharma A, Di Lello P, Benz J, D'Arcy B, Debulpaep M, Fry D, Huber W, Kremer T, Laeremans T, Matile H, Ross A, Rufer AC, Schoch G, Steinmetz MO, Steyaert J, Rudolph MG, Thoma R, Ruf A
J Biol Chem 291 16292-306 (2016)
PMID: 27226599

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-102762 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chains: A, L
Molecule details ›
Chains: A, L
Length: 213 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0U5BC76 (Residues: 22-233; Coverage: 99%)
Gene name: LC
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chains: B, H
Molecule details ›
Chains: B, H
Length: 215 amino acids
Theoretical weight: 22.8 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q569X1 (Residues: 21-240; Coverage: 47%)
Gene name: Ighg
Sequence domains:
Structure domains: Immunoglobulins
Neuronal migration protein doublecortin Chains: M, N
Molecule details ›
Chains: M, N
Length: 90 amino acids
Theoretical weight: 10.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43602 (Residues: 52-140; Coverage: 24%)
Gene names: DBCN, DCX, LISX
Sequence domains: Doublecortin
Structure domains: Doublecortin domain

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 66.858Å b: 110.399Å c: 75.092Å
α: 90° β: 106.54° γ: 90°
R-values:
R R work R free
0.201 0.198 0.265
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure-Guided Prediction of the Functional Impact of DCLK1 Mutations on Tumorigenesis.
Carli et al. (2023)
7 other articles

3 mentions without citation

Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies.
Burger et al. (2016)
2 more