› 5ih6

X-ray diffraction
2.3Å resolution

Human Casein Kinase 1 isoform delta (kinase domain) in complex with Epiblastin A derivative

Released:
DOI: 10.2210/pdb5ih6/pdb
PDB entry 5ih6 coloured by chain, front view.
PDB entry 5ih6 coloured by chain, side view.
PDB entry 5ih6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Epiblastin A Induces Reprogramming of Epiblast Stem Cells Into Embryonic Stem Cells by Inhibition of Casein Kinase 1.
Ursu A, Illich DJ, Takemoto Y, Porfetye AT, Zhang M, Brockmeyer A, Janning P, Watanabe N, Osada H, Vetter IR, Ziegler S, Schöler HR, Waldmann H
Cell Chem Biol 23 494-507 (2016)
PMID: 27049670

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [tau protein] = ADP + [tau protein] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155796 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase I isoform delta Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 34.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48730 (Residues: 1-294; Coverage: 71%)
Gene names: CSNK1D, HCKID
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand SRT 1 x SRT
Ligand SO4 2 x SO4
Ligand AUG 1 x AUG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 65.72Å b: 65.72Å c: 151.72Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.233 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Casein kinase 1α: biological mechanisms and theranostic potential.
Jiang et al. (2018)
12 other articles

6 mentions without citation

Past, Present, and Future Perspectives on Computer-Aided Drug Design Methodologies.
Bassani et al. (2023)
5 more