› 5i67

X-ray diffraction
2.6Å resolution

Crystal Structure Analysis of MTB PEPCK mutant C273S

Released:
DOI: 10.2210/pdb5i67/pdb
PDB entry 5i67 coloured by chain, front view.
PDB entry 5i67 coloured by chain, side view.
PDB entry 5i67 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Ra
Entry authors: Dostal J, Pachl P, Machova I, Pichova I, Snasel J

Function and Biology Details

Reaction catalysed: 
GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107722 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase [GTP] Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 67.18 KDa
Source organism: Mycobacterium tuberculosis H37Ra
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A5TYT6 (Residues: 2-606; Coverage: 100%)
Gene names: MRA_0219, pckG
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GDP 1 x GDP
Ligand PEG 3 x PEG
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: C2221
Unit cell:
a: 103.721Å b: 124.598Å c: 121.302Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.244
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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