› 5i5e

X-ray diffraction
1.62Å resolution

Crystal Structure of Transketolase mutants-H66/261C complex with xylulose-5-phoaphate from Pichia Stipitis

Released:
DOI: 10.2210/pdb5i5e/pdb
PDB entry 5i5e coloured by chain, front view.
PDB entry 5i5e coloured by chain, side view.
PDB entry 5i5e coloured by chain, top view.
Source organism: Scheffersomyces stipitis CBS 6054
Primary publication:
Structural and biochemical interrogation on transketolase from Pichia stipitis for new functionality.
Hsu LJ, Hsu NS, Wang YL, Wu CJ, Li TL
Protein Eng Des Sel 29 513-522 (2016)
PMID: 27578891

Function and Biology Details

Reaction catalysed: 
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transketolase Chain: A
Molecule details ›
Chain: A
Length: 697 amino acids
Theoretical weight: 74.98 KDa
Source organism: Scheffersomyces stipitis CBS 6054
Expression system: Escherichia coli
UniProt:
  • Canonical: P34736 (Residues: 1-677; Coverage: 100%)
Gene names: PICST_67105, TKT, TKT1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TPP 1 x TPP
2 bound ligands:
Ligand 5SP 1 x 5SP
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2221
Unit cell:
a: 99.885Å b: 182.084Å c: 98.263Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.158 0.182
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

The Mesomeric Effect of Thiazolium on non-Kekulé Diradicals in Pichia stipitis Transketolase.
Hsu et al. (2018)