› 5hu6

X-ray diffraction
2.9Å resolution

Structure of the T. brucei haptoglobin-haemoglobin receptor bound to human haptolgobin-haemoglobin

Released:
DOI: 10.2210/pdb5hu6/pdb
PDB entry 5hu6 coloured by chain, front view.
PDB entry 5hu6 coloured by chain, side view.
PDB entry 5hu6 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for ligand and innate immunity factor uptake by the trypanosome haptoglobin-haemoglobin receptor.
Lane-Serff H, MacGregor P, Lowe ED, Carrington M, Higgins MK
Elife 3 e05553 (2014)
PMID: 25497229

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-133189 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemoglobin subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chain: B
Molecule details ›
Chain: B
Length: 141 amino acids
Theoretical weight: 15.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P68871 (Residues: 3-143; Coverage: 96%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins
Haptoglobin beta chain Chain: C
Molecule details ›
Chain: C
Length: 259 amino acids
Theoretical weight: 28.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00738 (Residues: 148-406; Coverage: 67%)
Gene name: HP
Sequence domains: Trypsin
Haptoglobin-hemoglobin receptor Chain: D
Molecule details ›
Chain: D
Length: 260 amino acids
Theoretical weight: 27.93 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q581F2 (Residues: 38-297; Coverage: 71%)
Gene names: Tb06.28F21.220, Tb927.6.440
Sequence domains: Haptoglobin-hemoglobin receptor
Structure domains: Ferritin

Ligands and Environments

3 bound ligands:
Ligand HEM 2 x HEM
Ligand OXY 2 x OXY
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 222.29Å b: 56.56Å c: 66.1Å
α: 90° β: 92.97° γ: 90°
R-values:
R R work R free
0.192 0.19 0.226
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

5 review citations

Haptoglobin.
Andersen et al. (2017)
4 more

2 mentions without citation

Human Plasma Protein Corona of Aβ Amyloid and Its Impact on Islet Amyloid Polypeptide Cross-Seeding.
Nandakumar et al. (2020)
1 more